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Prosem Lecture: RNA-Sequencing Based Transcriptome Analysis of Differentially Expressed Genes in VF Epithelial Development in a Murine Model
October 1, 2018 @ 12:00 pm - 1:00 pm
Presenter: Vidisha Mohad, MS, CCC-SLP, PhD Candidate, Department of Communication Sciences and Disorders, Division of Otolaryngology, Department of Surgery, University of Wisconsin-Madison
Topic: RNA-Sequencing Based Transcriptome Analysis of Differentially Expressed Genes in VF Epithelial Development in a Murine Model
Understanding the molecular genetic mechanisms of vocal fold (VF) development is crucial however, little is known about VF embryonic development. The purpose of the study was to develop a comprehensive transcriptome profile of VF in a murine model. We used RNA-seq with Empirical Bayes analysis to identify early gene expression signatures that distinguish VF lineage from that of the adjacent trachea and esophagus lineage, as well as identify dynamic gene expression changes in VF as it progresses through development. We evaluated differential gene expression at stages embryonic (E) 15.5, post-natal day 0 (P0) and adult, as well as compared the gene expression between VF, trachea, and esophagus. Through enrichment analyses, we identified biological relevant pathways and gene ontology processes. Additionally, comprehensive cluster analysis has revealed association in defined biological processes during VF development. We validated expression of two key genes Nkx 2.5, and Nfatc2 between vocal fold, trachea, and esophagus. Our findings confirm that Nkx 2.5 and Nfactc2 genes are expressed in VF epithelium during E15.5 stage with a subsequent downregulation with developmental progression. We postulate that Nkx 2.5 could be key player guiding the reciprocal relationship between mesoderm induction of endoderm patterning during embryonic development.